Estimation of bacterial diversity in soil and vermicompost using sole source carbon utilization(SSCU) profile


Nipun Silawat1*, Shweta Chouhan1, Pramod Sairkar1, R. K. Garg1, Neetu Vijay2and N. N.Mehrotra3

In this research study, three samples were collected from the T.S. Murthi Udhyan, Obedullahganj out of which two samples were from agriculture field soil and the third sample was from compost. Pure cultures of bacteria were isolated by using serial dilution and spread plate methods. The isolated bacterial cultures were separated according to the morphotypes and then bio-chemically tested. They were further identified with the help of bacteria identification program (PIBWin 2007). The identified bacterial cultures are Achromobacter group F, Acinetobacter calcoacet, Pseudomonas aeruginosa, P. malli, P. putida, P. cepacia, P. shutzeri, P. pseudomalli, P. malli and P. pickettii. The value of Simpson’s index (D) is 0.09, which represented high diversity. Isolated bacteria were inoculated in micro titer plates and Niche overlap index (NOI) was calculated after two days of inoculation. The results of study indicate that direct incubation of samples in micro titer plates produces patterns of metabolic response useful in the classification and characterization of microbial communities. Principal component analysis of colour responses quantified from digitized images of plates revealed distinctive patterns among microbial habitats and spatial gradients within soil and compost sites. Correlation of the original carbon source variables to the principal components gives a functional basis to distinctions among communities. Intensive spatial and temporal analysis of microbial communities with this technique can produce ecologically relevant classifications of heterotrophic microbial communities.

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