Abstract

Naveed Akhtar , Shahiq-uz-zaman , Barkat Ali Khan *, Haji M , Shoaib Khan , Mahmood Ahmad , Fatima Rasool , Tariq Mahmood and Akhtar Rasul

The aim of this study was to determine the effects of newly formulated W/O emulsion (cream) of Calendula versus its vehicle (base) as control on skin pH, skin melanin, skin erythema, skin moisture content and transepidermal water loss (TEWL).Hydroalcoholic extract of Calendula plant was entrapped in the inner aqueous phase of the W/O emulsion. Base without active and formulation having 3% extarct of calendula in the aqueous phase were prepared. Samples were stored at different storage conditions that is, 8, 25, 40, 40°C+75% RH for four weeks to predict their stability. The newly formulated base and formulation were applied to the cheeks of 21 healthy human volunteers for a period of 8 weeks. The base showed insignificant (p>0.05) effects while the formulation decreased TEWL however this decrease was statistically insignificant (p> 0.05). Skin moisture content was significantly (p≤0.05) increased by the formulation. The base showed insignificant (p>0.05) effects while the formulation showed statistically significant (p≤0.05) decrease in sk?n melan?n content. Sk?n erythema was significantly reduced by the formulation. Skin sebum was significantly (p≤0.05) incresaed by both creams (base and formulation). Both creams were aesthetic with respect to sensory evaluation. The topical non-invasive application of Calendula officinalis cream showed a positive rejuvenating effect on human skin. This study will encourage more attention towards research and more conviction towards utilization of herbal medicines.