Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate host


Mohammed M. A. Al-Fayadh, Maani N. Al-Shemari and Ihsan E. Al-Saimary*

This research includes a study of hydatid cysts Echinococcus granulosus (larval stage on the molecular level, where 7 samples of hydatid cysts were collected from parasite intermediate hosts " Human (liver, spleen, lung) and liver of sheep, goat, cattle and buffaloes”. DNA was extracted from germinal layer cells of hydatid cysts which were isolated shortly or preserved for various periods in 70% ethanol. Genetic analysis of isolated DNA from hydatid cysts collected from human and animals was done by polymerase chain reaction (PCR) to determine genetic variation depending on random amplified polymorphic DNA. In the present study 10 primers have been used, during which the genetic variations were revealed among isolated (extracted DNA) of hydatid cysts which was collected from human and other intermediate hosts except Cows and Buffaloes. The current results of this study have shown the following: 1 - It was found one primer (OPA – 01) was able to diagnose sample numbered 1 which represent the isolated DNA of liver hydatid cyst which was obtained from human at age group 10  – 20 years old. 2 - The ability of primer OPC – 10 to determine fingerprinting of DNA sample of Sheep liver hydatid cyst. 3 - The ability of primer OPC – 05 to determine fingerprinting of DNA sample of human spleen hydatid cyst which was obtained from human at age group 30 – 40 years old. 4 - The ability of primer OPE – 07 to determine fingerprinting of DNA sample of goat liver hydatid cyst. 5 – Amplification process to the DNA samples which extracted from cows and buffaloes liver hydatid cysts was not completed by using all 10 primers. 

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