Screening and optimization of nutrients for Lasparaginase production by Bacillus cereus MNTG-7 in SmF by plackett- burmann design


M. Sunitha*, P. Ellaiah and R. Bhavani Devi

The medium components influencing the enzyme production were screened using Plackett-Burman design. A total of 67 nutrients comprising of fifteen each of carbon, nitrogen, mineral/salt and eleven each of inorganic nitrogen sources and amino acid sources were screened for the production of Lasparaginase by SmF. This design involves screening of up to ‘n-1’ variables in just ‘n’ number of experiments. Regression coefficients and t-values were calculated by subjecting the experimental data to statistical analysis. Among the 67 nutrients based on their performance in terms of product yield and cost, tapioca starch, L-asparagine, ammonium oxalate, gelatin and CaCO3 were identified as most effective and therefore selected for inclusion in surface methodology studies.

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