Direct detection of Brenneria rubrifaciens by (polymerase chain reaction) PCR-based assay using rubrifacine synthetic gene


Shree Prasad Thapa, Chun Keun Lim, Sam Kyu Kim, Jun Mo Cho, Jang HyunHur and Duck Hwan Park

A rapid high-throughput, specific and sensitive (polymerase chain reaction) PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogen Brenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that the specific genetic markers have a powerful potential to detect B. rubrifaciens directly from crude samples at field conditions. Key words: B

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