Genotyping of bovine viral diarrhea virus using multiplex PCR, with and without RNA extraction


Kawther S. A. Zaher

Bovine viral diarrhea virus (BVDV) is a very important viral disease, which constitutes a major problem worldwide due to the carrier state and due to its misdiagnosis with other viruses. The goal of the current study was to detect and serotype BVDV through multiplex PCR with and without RNA extraction, due to the fact that extraction of RNA may be laborious. Blood samples were taken from 100 randomly-selected animals, containing diseased animals suffering from diarrhea and respiratory manifestation. One part of the sample was subjected to RNA extraction and the other part was not, before performing reverse transcriptase (RT)-PCR. The assay succeeded to type BVDV with and without extraction RNA, as well as in detecting carrier animals. BVDV-2 was detected in a slightly higher number of animals than BVDV-1 (27 and 25, respectively), while 47 buffalo were identified as carrier animals.

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