High level production of the recombinant gag24 protein from HIV-1 IN Escherichia coli.


Ramón E. Narciandi Díaz1 *, José Motolongo Viquillon2 , Luis Pérez Figueroa2 .

The gag24 gene of the Human Immunodeficiency Virus type 1 (HIV-1) was expressed under the control of the tryptophan promoter in Escherichia coli. The effect of several parameters on the production of gag24 was studied. The expression level achieved (25%) depended on the host strain and the induction conditions. The developed fermentative process was scaled-up to 50 L, where 417 mg/L of volumetric production and 34 mg/L.h of productivity, were obtained.

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