Occurrence of tetracycline-resistant Aeromonas hydrophila infection in Korean cyprinid loach (Misgurnus anguillicaudatus)


Jin Woo Jun, Ji Hyung Kim, Dennis K. Gomez, Casiano H. Choresca Jr, Jee Eun Han, Sang Phil Shin and Se Chang Park*

In this paper, we described a mass mortality of cyprinid loaches, Misgurnus anguillicaudatus from a private fish farm in Korea. Diffuse bleeding was observed on the skin around the anal orifice. Bacterial pathogens from kidneys samples of moribund fish were cultured, identified and confirmed to be Aeromonas hydrophila using morphological, biochemical test and genetical analysis. The isolated A. hydrophila strains were resistant to commercial antibiotics and the presence of the tetracycline resistance gene (tet E) was detected by genetical analysis. Pathogenecity test was performed using healthy 4-week-old cyprinid loaches by intraperitoneal (IP) injection and the LD50 concentration of the pathogen was determined to be 6.0 × 107 CFU fish-1 . In this paper, we confirmed the infection of farmraised cyprinid loaches with tetracycline-resistant A. hydrophila.

Share this article

Awards Nomination

Select your language of interest to view the total content in your interested language

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Directory of Open Access Journals
  • CiteFactor
  • Electronic Journals Library
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Publons
  • PubMed
  • Rootindexing
  • Chemical Abstract Services (USA)
  • Academic Resource Index