Production of poly- -hydroxybutyrate (PHB) and differentiation of putative Bacillus mutant strains by SDS-PAGE of total cell protein ..


Hikmet KatırcıoÄ?lu1*, Belma Aslım2, Zehra Nur Yüksekdað2, Nazime Mercan3, Yavuz Beyatlı2

In this study, the putative mutant strains of Bacillus megaterium Y6, B. subtilis K8, B. sphaericus X3 and B. firmus G2 were studied for their poly- -hydroxybutyrate (PHB) production capacities. Mutations were induced by using UV light, acriflavin and 5-bromourasil. Total cell proteins were extracted from 59 strains and compared using SDS-PAGE. For each strain, percentage yield of PHB according to cell dry weight was determined in a range of 1.46-63.45%. PHB production of 8 mutant strains were found to increase in comparison with parental strains. However, no increase in PHB production of mutant strains of B. sphaericus X3 was found. It was also determined that the protein profiles of the mutant strains with high PHB yield generally differed from the protein profiles of parental strains.

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