Temperature effect on dextransucrase productionby Leuconostoc mesenteroides FT 045 B isolated from Alcohol and Sugar Mill Plant..


Mariana CORTEZI1, Rubens MONTI2and Jonas CONTIERO1*

Temperature (23 to 31�?�C) and sucrose concentration (3 and 4%) effects on dextransucrase production by Leuconostoc mesenteroides NRRL B 512 (F) and Leuconostoc mesenteroides FT 045 B were studied. The conditions in all fermentations were: total reaction volume 2 L, 132 rev. min-1, 0.5 vvm and pH 6.0. The optimum temperature for enzyme yield for strain NRRL B 512 (F) was 23�?�C, where at 8-h fermentation was possible to achieve 49.3 DSU/mL. When FT 045 B strain was utilized, 3.2 DSU/mL was obtained at temperature 23 to 25 �?�C.

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