Abstract

U. Rani* R. N. Verma and A. Batra

In the present study, antioxidant activities of the three plant parts (stem, leaf and callus) were investigated. Ethanolic extracts of stem, leaf and callus powder was characterized by the method described by (Hatano et al., 1988) in which stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical solution was used. In this paper, it is found that all of the in vitro and in vivo extracts have remarkable antioxidant activities. The IC50 (inhibition concentration 50) of the ethanolic extracts of M Koenigii callus-stem-leaf were 47-78, and 116 μg/ml respectively. The total phenolics and flavonoid content of the Murraya koenigii callus cultures were also measured. Among them in vitro callus showed higher antioxidant activity, due to phenolics and flavonoid content. Therefore, the research clearly indicates that M. koenigii callus is exceptionally advantageous for human health.