Xylanase production by Penicillium chrysogenum (PCL501) fermented on cellulosic wastes.


Okafor, U. A.1 , Emezue, T. N.1 , Okochi, V. I. 1 ; Onyegeme-Okerenta, B. M. 1 and Nwodo-Chinedu, S.2

Xylanase production by Penicillium chrysogenum PCL501, newly isolated from wood-wastes, was monitored at 24 h intervals for a period 168 h in media containing four different carbon sources (oatspelt xylan, wheat bran, sawdust, and sugarcane pulp). The highest xylanase activity of 6.47 Units mL-1 was obtained at 96 h in media containing wheat bran whereas media containing sugarcane pulp gave a peak value of 1.39 Units mL-1 at 144 h. Sawdust and xylan gave a peak xylanase activity of 1.35 and 0.79 Units mL-1 respectively at 120 h. Maximum protein released in xylan-containing media was 0.38 mg mL1 . Higher protein yield was obtained in media containing the agro-wastes, with wheat bran giving the highest value of 1.14 mg mL-1 . The maximum specific xylanase activities were 2.59, 8.52, 16.06, and 9.36 Units mg Protein -1 for sawdust, sugarcane pulp, wheat bran and xylan respectively. Out of the three agro-wastes used in this study, wheat bran holds the greatest promise for cost -effective production of the xylanase enzyme. The carbon source is the highest inducer of the enzyme in the fungus

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