Abstract

Byung Uk Kim , Mi Ae Jeong, Yeon Sun Ryu , Hwa Chun Park , Jong Hyun Jung , Sam Woong Kim , Chul Wook Kim , Young Min Song , Ki Hwa Chung, Il Suk Kim , Sang Keun Jin , Sang Suk Lee , In Soon Choi and Kwang Keun Cho

Proteomic analysis by 2-dimensional electrophoresis (2-DE) of the longissimus dorsi muscle in finishing pigs (LY × D) grown on a diet supplemented with mugwort powder found roughly 300 spots on a polyacrylamide gel image. Among them, the expression levels of 12 spots were higher than those of the control group. From the results of peptide mass fingerprinting (PMF) analysis, nine proteins were characterized: myosin light chain 2V, elF-5A, myosin light chain 1, F1- ATPase chain D, peroxiredoxin-2, Kelch-related protein 1, SLA-7, glycine amidinotransferase, and Tpi 1. The other three spots with increased expression were identified by chemically assisted fragmentation-matrix-assisted laser desorption/ionization (CAF-MALDI) sequencing as serum albumin precursor and two different types of myoglobin. Taken together, these results indicate that mugwort powder has the potential to improve the quality of pork meat.