Abstract

Onami Steyn, Keabetsoe T. and Thabo Moreki

Anthurium andraeanum is an important ornamental in the flower industry in Mauritius. Classical phenotype methods of identification, although still very useful, are difficult to use between very closely related Anthurium cultivars. The objectives of this study were to assess the genetic diversity of 12 A. andraeanum cultivars in Mauritius using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers and identify cultivar specific markers for the genetic profiling of A. andraeanum cultivars. Polymorphism among the 12 cultivars was assessed using RAPD and ISSR primers. Reproducible results were used for statistical analysis. The presence and absence of bands were scored as 1 and 0, respectively to form a matrix from which a dendrogram was obtained using NTSYS. Dendrograms were obtained from matrices derived from RAPD and ISSR analyses to give an estimate of the genetic distance between the tested cultivars. Both ISSR and RAPD were found to be useful tools in differentiating locally grown A. andraeanum.