Abstract

Angora KE, Ako AB, Bosson-Vanga AH, Ira-Bonouman AV, Touré OA, Menan HE and Djaman AJ

Schistosoma haemtobium infection remains an important place in neglected tropical diseases in subSaharan Africa. DNA detection of this parasite is never done in Côte d'Ivoire; so it is important to carry out this study which aims to perform phenol-chloroform method S. haematobium DNA extraction from urine in Parasitology Unit of Institut Pasteur. Thus, 107 urine samples positive for S. haematobium stored at -20°C collected from schoolchildren in Guebo 2 were used. Optical densities (OD) were measured by spectrophotometer at 260 nm and 280 nm wavelength and DNA concentrations were measured. The average of optical density was 0.780 ± 0.61 at 260 nm and 0.687 ± 0.53 at 280 nm. Most extracts DNA had OD260nm / OD280nm ratio lower than 1.8. DNA concentrations were high with an average of 234.0 ± 184.3μg / mL with extremes at 33.7μg / mL and 827.5μg / mL. Phenol-chloroform method gave relatively high concentrations of DNA. The PCR amplification of DNA will allow improving this method.