Abstract

S. Parvez*, Z. Mukhtar, F. Rashid, M.I. Rajoka

A β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae. Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicating that the gene is expressed in the bombarded yeast.