Agronomic and molecular evaluation of recombinant inbred lines (RILs) of lentil.

Abstract


C. Bermejo, V. P. Cravero, F. S. López Anido and E. L. Cointry

Sequence-related amplified polymorphism (SRAP) and morphological markers were studied to compare the efficiency of both marker systems in the evaluation of twenty five lentil (Lens culinaris Medik.) recombinant inbred lines (RILs) and four testers. Data on 13 morphological traits were collected and analyzed. A total of 240 polymorphic SRAP’s bands (76.7%) were scored using four combinations of primers. Cluster analysis and both principal component and principal coordinate analysis were carried out. The entries were grouped in five clusters through procrustes generalized analysis. Relationships among lines revealed by molecular markers were significantly correlated with those based on the agronomic traits (r = 0.75), suggesting that the two systems give similar estimates of genetic relations among the RILs. In future breeding programs parent selections could be based on these traits information in order to broaden the genetic.

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