Abstract

Syed M. Usman Ali , M. Fakhar-e-Alam , Z.Wazir ,M. Kashif , M. Atif , Magnus Willander and W. A. Syed

Cytotoxicity of bare and conjugation of Tween-80 capsulated nanomaterials e.g. zinc oxide nanoflakes (ZnO NFs), iron oxide nanoparticles (Fe2O3 NPs) was examined in dark as well as under light exposure in cell model of immature human muscle carcinoma (RD) via microinjection and free standing drug delivery system. ZnO NFs were grown on the tip of a capillary and characterized by applying atomic force microscopy (AFM) technique and the tip was used as pointer to insert chemicals into cell to visualize/assess the emission of reactive oxygen species (ROS) especially from mitochondria. Bare and conjugated ZnO NFs with δ-aminolevulinic acid (ALA) were irradiated /excited with UV light after cellular uptake, reactive oxygen species were generated. We deduct that ROS is damaging mitochondria resulting in cell necrosis within few minutes. ZnO NFs are not biosafe and have significant toxic effects for both normal as well as cancer cell especially for rhybdomyosarcoma cell line (RD).