Detection of the antibiotic resistance genes in Staphylococcus aureus isolated from human infectionsand bovine mastitis

Abstract


Malihe Heidari1*, Hassan Momtaz2and Mahboobeh Madani

The present study was carried out in an attempt to detect the distribution of antibiotic-resistant genes of Staphylococcus aureusisolates from human infections and bovine mastitis. mecA, msrA, msrB,aacA-D, tetK andtetM genes were selected in order to detect the distribution of antibiotic-resistantgenes by multiplex PCR technique. According to the biochemical analysis and detection of 16S-rDNA by PCR method, 108 isolates of 300 human infections samples and 18 strains from 150 bovine mastitis milks were recognized as S. aureus. Distributions of antibiotic-resistant genes in human isolates were as follows: (85.18%) mecA, (46.29%) msrA, (49.07%) msrB, (33.33%) aacA-D, (80.50%) tetKand (66.66%) tetM and in bovine mastitis, isolates were seen to be ranging from: (22.22%)mecA, (66.66%)msrA,(77.77%) msrB, (33.33%) aacA-D, (55.55%) tetKand (50.00%) tetM, respectively. Results indicated that all S. aureus strains have one or more of the antibiotic-resistant genes. Also, multiplex PCR technique is afast, practical and appropriate technique for determining antibiotic-resistant genes. Hence, it was possible that the treatment and the right antibiotics were used

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