First identification of ba.5.2.1 omicron sub-lineages in fully vaccinat- ed patients in Cameroon


Martin Maïdadi Foudi, Célestin Godwe, Emilande Guichet, Nicolas Fernandez Nuñez, Guy-Roland Awoundza, Nadine Lamare, Marie Amougou Atsama, Marcel Tongo, Onana Boyomo, Wifred Mbacham, Ahidjo Ayouba, Martine Peerters, Eric Delaporte and Charles Kouanfack*

Omicron is the most mutated SARS-CoV-2 variant; its high transmissibility and immune evasion ability have raised global concerns. We reported here the first identification of BA.5.2.1 Sub-lineage of Omicron variant in two fully vaccinated patients in Cameroon. We used DICOV-MOC/I MULTIPLEX RT-PCR and CovidSeq on Illumina ISeq100 respectively for the screening of SARS-CoV-2 mutations and whole-genome sequencing. We detected antibodies to Spike (SP) and Nucleocapsid (NC) proteins from ancestral and Omicron SARS-COV-2 strains using a multiplex Luminex-based assay on a MagPix platform. On the 15th of June 2022, two patients (Cases 1 and 2) presented at CREMER laboratory in Yaoundé for COVID-19 test. Nasopharyngeal Swab RT-PCR results were positive with Treshold Cycle of 16.7 and 19.3 for both the cases respectively. Mutations screening revealed the presence of K417N and L452 mutations for Case 1 and a single K417N mutation detected for Case 2. We obtained two full-length of SARS-CoV2 genome. Phylogenetic analysis showed that the two strains belonged to the BA.5.2.1 sublineage of Omicron. Serological analysis of Case 1 plasma collected 25 days after the onset of symptoms showed high levels of antibodies against SP and NC of ancestral and Omicron variants.

Share this article

Awards Nomination

Select your language of interest to view the total content in your interested language

Indexed In
  • Index Copernicus
  • Sherpa Romeo
  • Open J Gate
  • Academic Keys
  • CiteFactor
  • Electronic Journals Library
  • OCLC- WorldCat
  • Academic Resource Index