Metabolite profiling of endophytic fungal isolates of five ethno-pharmacologically important plants of Meghalaya, India


R. K. Bhagobaty and S. R. Joshi *

Crude metabolite extracts derived from the culture broths of endophytic fungi of five ethno-pharmacologically important plant species used by the ethnic tribes of the hill state of Meghalaya in North-East India were screened for the presence of toxins and secondary metabolites using agar plug paper chromatography, thin layer chromatography (TLC) and liquid chromatography-mass spectroscopy (LC-MS). The endophytic fungal isolates were found to produce a wide range of toxins, growth hormones and antibiotics upon searching in a fungal mycotoxin database. Relatedness among the fungal isolates was determined based upon their extra-cellular metabolite production pattern. The endophytic fungal isolates in the present study showed a wide range of metabolomic diversity and can be explored as potential microbial cell factories for production of a diverse range of biomolecules. Taxonomic relatedness of the endophytes based upon the snapshots of their secretomes, presented a contrasting picture in relation to their morphological identity. Rapid metabolome analysis of endophytic fungi using analytical techniques like LC-MS coupled with mass related search in fungal specific metabolite databases is bound to increase our insights about fungal chemo-taxonomy as a whole and bio-prospection of useful fungal metabolites in particular.

Share this article

Awards Nomination

Select your language of interest to view the total content in your interested language

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Academic Keys
  • CiteFactor
  • Electronic Journals Library
  • OCLC- WorldCat
  • Leipzig University Library
  • WZB
  • ZB MED
  • Bibliothekssystem Universit├Ąt Hamburg
  • German National Library of Science and Technology
  • Universitat Des Saarlandes Library
  • Academic Resource Index