Reverse transcriptase polymerase chain reaction is a sensitive diagnostic tool for detection of nodavirus infection in Macrobrachium rosenbergii.

Abstract


D. P. Behera, K. Devdas and L. Nayak

Macrobrachium rosenbergii is the most important cultured Palaemonid in the world. It is farmed on a large scale in many countries including India, once upon a time this species was considered as disease free animal but towards later part of 2001, the hatchery and nursery pond of these animals suffered a massive loss due to a new viral disease named white tail disease. Till date different diagnosis has been established for detection of this disease but reverse transcriptase polymerase chain reaction (RT-PCR) techniques is the most sensitive diagnostic tool among those present available for detection of MrNv (Macrobrachium rosenbergii nodavirus). The designed primer fair (1F/1R) in 1st step PCR was carried out using 1 µl e DNA like wise in 2nd step RT-PCR product the nested primer 2nd 1 µl of 1st RT-PCR product were utilized. At the end of 8 µl of each step PCR product was analyzed by electrophoresis on 1% agarose gel along with sample. The confirm positive and negative sample were run in the gel, the gel electrophoresis of RT-PCR product positive control (Lane-3) is expected amplified product of about 489 bp size of noda virus presence in sample.

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