Nathan C Incandela

Department of Chemistry and Biochemistry, University of California, Los Angeles, USA

Publications

• Research Article   
  Characterizing the antibody specificity of sera from malaria endemic African countries for sequence micro-heterogeneities within the dimorphic domain of Pf3D7-MSP2
  Author(s): Saidou Balam*, Nathan C Incandela, Drissa Konate, George Agak, Mahamadou Diakite, Remy Moret, Issa Nebie and Giampietro Corradin
  
  Background: Micro-heterogenicity of 25-mer segments within the dimorphic domain (Pf3D7-D) defined various alleles of Pf3D7-type MSP2. This study explored the specificity of African malaria-endemic antibodies (IgG) to this micro-heterogenicity.   Methods: We tested in ELISA immunoassays (standard and competitive ELISA) 12 synthetic 25-mer   peptides representing each micro-heterogenous segment using sera from Mali, ML (N=75), Burkina Faso, BF (N=49) and Tanzania, TZ (N=37).   Findings: Overall, BF antibody samples had significantly higher affinities (p<0.0001) and the largest proportion (45%-65%) of positive responders for all the 25 mer-peptides. In contrast, ML samples had slightly lower antibody affinities and a smaller proportion of responders (15%-57%), whereas TZ sample demonstrated the lowest anti.. View More»
  

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