A study of the optimum culture conditions and factors involved in maximum production of glucoamylase

Abstract


Lagzouli Mohamed*, Mennane Zakaria , Aitounejjar Ali, Wafae Senhaji,Ouhssine Mohamed, Elyachioui Mohamed, Berny EL Hassan and Jadal Mohamed

Candida famata was isolated from traditional Moroccan sourdough. It exhibited high glucoamylase and biomass production. Starch induces high glucoamylase production C. famata with maximum glucoamylase activity at 5 g/L. Glucose stimulates good production in biomass but strongly inhibits glucoamylase production. Among the sources of nitrogen tested, yeast extract and the (NH4)2HPO4 gave maximum glucoamylase and biomass after 72 h of incubation in liquid medium at 30°C, pH 5 and 105 rpm.

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