Abstract

Purwati Armand, Fedik. A. R, Sony Wibisono, Anas. P, Eric. H, Helen. S, Deya. K

Alternative therapies in diabetes mellitus (DM) management include the use of stem cells. Stem cells derived from the bone marrow and pancreatic cells from allogenic donors were used in this experimental animal model to restore glucose control. DM was induced in Wistar rats with 50 mg/kg alloxan. DM rats were divided into 4 treatment groups, Group 1 was transplanted with autologous bone marrow derived mesenchymal stem cells (MSC) by intraperitoneally injection. Group 2 was given allogenic pancreatic cells intraperitoneally. Group 3 was given insulin subcutaneously, and Group 4 served as control (no treated). The dosage was 200,000 cells/rat. Post therapy results in group 1 revealed significant decrease of blood sugar levels, an increase in insulin levels, and increased C peptide levels. In group 2, there were more pronounced changes, improvements in glucose control compared to group 1. Those receiving only insulin the levels of blood sugar decreased but less so compared to those receiving MSC or pancreatic cells (p = 0.002). In a DM Wistar rat model the intraperitoneal administration of pancreatic cells resulted in better restoration of glucose control than intraperitoneally of bone marrow derived MSC, which in turn was better than only insulin.