IN VITRO explants regeneration of the grape ???Wink??? (VITIS VINIFERA L. ???Wink???)


Ping Zhang, Zhi-Ying Yu, Zong-Ming Cheng, Zhen Zhang and Jian-Min Tao*

The effects of different hormones and their concentration combinations, different explant types, dark periods, on the regeneration of the grape ‘Wink’ was investigated using IN VITRO leaves, petioles, internodes and radicles. The results showed that of all the media prepared, the explants on following media produced the highest regeneration rate: 78.74±1.60% of the leave explants on the medium prepared with MS+18.20 µM, TDZ+0.49 µM IBA regenerated with a mean of 6.75±0.75 shoots per explant; 39.33±1.47% of the petioles on the medium prepared with MS+9.10 µM TDZ+0.49 µM IBA regenerated with a mean of 3.55±0.50 shoots per explant and 41.37±1.13% of internodes on the same medium regenerated with a mean of 4.74±0.64. However, radicles did not generate on any of the media. TDZ enhanced regeneration rate of leaves better than BA. From 0 week to 4 weeks in dark, leave explants displayed a higher rate of regeneration for 2 or 3 weeks. Adventitious shoots were rooted on 3/4 MS+1.73 µM IBA medium, and the rooted plantlets survived after acclimatization and they were transplanted to the greenhouse.

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