Kathleen L Hefferon

Department of Microbiology, Cornell University, Ithaca, USA

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  • Research   

    Author(s): Zahida Qamar*, Idrees Ahmad Nasir, Mounir G Abouhaidar, Kathleen L Hefferon, Muhammad Ahmed and Ahmad Ali Shahid

    Due to higher amount of sucrose, sugarcane is grown commercially. In order to save sucrose yields, various studies have been designed to develop resistance in sugarcane against weeds and stemborers. In this study, two problems had been addressed by genetic manipulation of sugarcane to make them resistant against both herbicides and insects by expressing glyphosate resistant gene (CEMB-GTGene) and borer resistant genes (CEMB-Cry1Ac and CEMB-Cry2A) under control of Nos terminator and maize ubiquitin promoter. Mortality percentage of shoot borers Chilo infuscatellus was determined by assessing the Cry proteins through insect Bio-toxicity assays. Results showed that in 80 days old transgenic plants, 100% mortality rates of Chilo infuscatellus have been found showing that there was high resistance in transgenic sugarcanes against shoot borers and sufficient gene expression to fully resist .. View More»

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