An efficient protocol for in vitro clonal propagation of natural sweetener plant (Stevia rebaudiana Bertoni)


Ashish Ojha

Leaf segments of Stevia were cultured on MS medium supplemented with varying concentrations (0.5, 1.0, 1.5, 2.0, 2.5 mg/L-1 ) of growth regulators (BAP and 2, 4-D). Ninety one percent aseptic cultures were obtained when sterilized with 0.1% HgCl2 for 10 min. The highest amount of callus was obtained in MS medium supplemented with1.0 mg/L-1 BAP+0.5 mg/L-1 2, 4-D, respectively. On the other hand, 2.5 mg/L1 BAP + 0.5 2,4-D showed lowest performance. Highest shoot was obtained in 2.0 mg/L-1 BAP. These shoots was transfer into different concentration of IBA, NAA and IAA (0.5. 1.0, 1.5, and 2.0) used. Highest rooting percentage was recorded on MS medium with 0.5 mg/L-1 IAA. The rooted plantlets were transfer into mist chamber with relative humidity for 2 - 4 week after these plants were hardened and successfully established in soil.

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